Prevalence of Hyper virulence and Multidrug resistance Klebsiella pneumoniae isolates from UTI patients
Prevalence of Hyper virulence and Multidrug resistance Klebsiella pneumoniae isolates from UTI patients
DOI:
https://doi.org/10.24237/ASJ.03.01.810BKeywords:
K. pneumoniae, FimH, rmpA, bla TEMgenes, Biofilm formation, Extended spectrum Beta-Iactamase (ESBL) production, siderophore production.Abstract
Urinary tract infections (UTIs) are the most common bacterial infections and are often caused by Klebsiella pneumoniae. the results of detection of some virulence factors 30(13.6%) isolates of K. pneumoniae showed that Hemolysin production was tested using sheep blood agar and it was found that all the 30 (100%) isolates of K. pneumonia not capable to hemolysis red blood. Capsule production was also investigated using nigrosin stain followed by direct microscopy examination and it was found that 28 (93.3%) from K. pneumoniae isolates were surrounded by a capsule as well as (100%) biofilm formers. 86.6%, 77% and 70% of the isolates were siderophore, protease and coagulase were giving positive results respectively. Biofilm production was tested using methods: micro-titer plate (MTP) method, the results showed 16 (53%) of K. pneumoniae were biofilm-former isolate The resistance to antibiotics was done and the results illustrated that the resistance toward ticarcillin-clavulante, cefotaxime, ceftriaxone and ciprofloxacin were 100%. while tetracycline and trimethoprime/sulfamethazole was 62% and streptomycin and norfloxacine was 51.7% and 48.2% respectively, while gentamicine was 44.8%, while impenem and meropenem was 37.9% and 6.8% respectively. Resistance patterns for the isolates K. pneumoniae was also calculated and 16(53%) isolates were MDR which includes isolates that have resisted three groups of antibiotics and the 14(47%) were XDR which resisted four or five groups of different antibiotics. The minimum inhibitory concentration (MIC) was determined for a total of 30 isolates of K. pneumoniae. Ceftazidime and cefepime were used as antimicrobial agents, and the measurements were conducted on Muller-Hinton agar. Serial dilutions were generated, and the resulting MIC values were found to be 512 and 1024µg/mL, respectively. The production of extended-spectrum beta-lactamases (ESBLs) was assessed through the utilisation of the double disc synergy test. Out of the total isolates of K. pneumoniae, 18 (60%) were identified as ESBL producers, while the remaining 12 (40%) were found to be non-producers. Molecular detection was carried out to detect some virulence genes in K. pneumoniae. Polymerase chain reaction was used to detect the adhesion gene fimH, results showed that (75%) of k. pneumoniae isolates had fimH, rmpA gene responsible for capsule formation and it was found in (66%) of K. pneumoniae, according to bla- TEM gene encoding ESBL, (71.2%) K. pneumoniae isolates had bla-TEM gene.
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