Correlation of Phylogenetic with Antibiotics Resistance Profiles and Integron Class I in Clinical Isolates of Klebsiella pneumoniae in Baquba City

Abstract

Klebsiella pneumoniae is a Gram-negative bacterium that causes serious infections and is
resistant to various antibiotics. Fifty isolates of Klebsiella pneumoniae were reactivated and
reidentified based on Compact System Vitek 2. All the isolates were tested for six antimicrobial
agents using the agar diffusion process. The final results were as follows: Ampencillin 96%,
Imipenem 38%, Cefotaxime 96%, Tobramycin 48%, Doxycycline 90%, and Azythromycin
66%. β--lactamase producers (MBL and AmpC) appeared at 70% and 22%, respectively.
Clinical K. pneumoniae isolates' RAPD amplification information revealed that 16 isolates were
0% Applying the integrase gene as an indicator for class 1 integron research and PCR analysis
revealed that 16 (100%) of K. pneumoniae Integrin I dendrograms of K. pneumoniae isolates
showed two main clusters: A and B. According to our data, resistance to all antibiotic classes
was shown to be more widespread in B lineages (100%), followed by A phylogenetic groupings,
which were resistant (100%) to cefotaxame and ampencillin.