Effecting of Ribosomal-Inactivating Proteins on Gene Expression of Cdr2 Gene in Pathogenic Candida Albicans Isolates
DOI:
https://doi.org/10.24237/ASJ.02.01.725DAbstract
Twenty isolates of Candida albicans were collected, during the study period from December / 2020 to February / 2021. For a group of 20 married women suffering from vaginal infections in Baqubah city. All the isolates were multidrug resistance against 5 antifungals from different groups according to the susceptibility test. The result of minimum inhibition concentration (MIC) for antifungal (fluconazole) values were between (256-64) µg/ml and MIC values for ribosomal inactivating proteins, between 325–5200 µg/ml . Conventional PCR technique was used to detect the CDR2 gene that is a molecular marker of a functional resistance for azoles antifungals group, the results inducted the presence of CDR2 gene 100%, it presented in the 20 isolates. By using Real-time PCR technique the CDR2 gene gave a low expression after treatment (32)µg/ml with sub MIC concentration of fluconazole with average folding 0.1once and after treatment 325 µg/ml with sub MIC ribosomal inactivating proteins concentration again of compared with control with average folding 0.6 in different degrees in two isolates were multi-resistant to antifungals.
References
Gajdács, M., Dóczi, I., Ábrók, M., Lázár, A., & Burián, K. (2019). Epidemiology of candiduria and Candida urinary tract infections in inpatients and outpatients: results from a 10-year retrospective survey. Central European journal of urology, 72(2), 209.
Sobel, J. D. (2007). "Vulvovaginal candidosis." Lancet 369(9577): 1961-1971.
Blostein, F., Levin-Sparenberg, E., Wagner, J., & Foxman, B. (2017). Recurrent vulvovaginal candidiasis. Annals of Epidemiology, 27(9), 575-582.
Baldewijns, S. (2021). Investigation and in vivo validation of the anti-Candida activity of probiotic metabolites in the vaginal niche (Doctoral dissertation, KU Leuven).
Ford, C. B., Funt, J. M., Abbey, D., Issi, L., Guiducci, C., Martinez, D. A., ... & Regev, A. (2015). The evolution of drug resistance in clinical isolates of Candida albicans. elife, 4, e00662.
Bongomin, F., Gago, S., Oladele, R. O., & Denning, D. W. (2017). Global and multi-national prevalence of fungal diseases—estimate precision. Journal of fungi, 3(4), 57.
Kably, B., Launay, M., Derobertmasure, A., Lefeuvre, S., Dannaoui, E., & Billaud, E. M. (2022). Antifungal drugs TDM: trends and update. Therapeutic Drug Monitoring, 44(1), 166-197.
Rello, J., Parisella, F. R., & Perez, A. (2019). Alternatives to antibiotics in an era of difficult-to-treat resistance: new insights. Expert Review of Clinical Pharmacology, 12(7), 635-642.
Wani, S. S.; Dar, P. A.; Zargar, S. M. and Dar, T. A. (2020). Therapeutic Potential of Medicinal Plant Proteins: Present Status and Future Perspectives.Current Protein and Pept. Sci., : 21( 5): 443-487.
Luo, S., Coutinho, B. G., Dadhwal, P., Oda, Y., Ren, J., Schaefer, A. L., ... & Tong, L. (2021). Structural basis for a bacterial Pip system plant effector recognition protein. Proceedings of the National Academy of Sciences, 118(10), e2019462118.
Weise, C.; Schrot, A.; Wuerger, L. T .; Adolf, J.; Gilabert Oriol, R.; Sama, S.; Melzig, M. F. and Weng, A. (2020). An unusual type I ribosome inactivating protein from Agrostemma githago L. Scientific Reports ,10:15377
Pizzo, E., Pane, K., Bosso, A., Landi, N., Ragucci, S., Russo, R., ... & Di Maro, A. (2018). Novel bioactive peptides from PD-L1/2, a type 1 ribosome inactivating protein from Phytolacca dioica L. Evaluation of their antimicrobial properties and anti-biofilm activities. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1860(7), 1425-1435.
Marinho, S. A., Teixeira, A. B., Santos, O. S., Cazanova, R. F., Ferreira, C. A. S., Cherubini, K., & Oliveira, S. D. D. (2010). Identification of Candida spp. by phenotypic tests and PCR. Brazilian Journal of Microbiology, 41, 286-294.
Park, S.W.; Prithiviraj, B.; Vepachedu, R. and Vivanco, J. M. (2006). Isolation and Purification of Ribosome-Inactivating Proteins. Methods Mol. Biol., 318:335-47
Bradford, j .A. and Heath, E. C. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein -dye binding. Analytical. Bioch., 72:248 -254.
Weinstein, M.P. and Lewis, J.S. (2019). Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100 (ISBN 978-1-68440-066-9 [Print]; ISBN 978-1-68440-067-6 [Electronic]). Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA:47..
HANCOCK, Robert EW; CHAPPLE, Daniel S. (1999) Peptide antibiotics. Antimicrobial agents and chemotherapy, 43.6: 1317-1323.
Zhang, M., Yan, H., Lu, M., Wang, D., & Sun, S. (2020). Antifungal activity of ribavirin used alone or in combination with fluconazole against Candida albicans is mediated by reduced virulence. International journal of antimicrobial agents, 55(1), 105804.
Schmittgen T and Livak K (2008) Analyzing real-time PCR data by the comparative (CT) method. Nat. Protoc. 3, 1101–1108.
Bhavan, P. S., Rajkumar, R., Radhakrishnan, S., Seenivasan, C. and Kannan, S. (2010). Culture and identification of Candida albicans from vaginal ulcer and separation of enolase on SDS-PAGE. Inter. J. Bio. 2 (1): 84-93.
Alshaikh, N. A., & Perveen, K. (2021). Susceptibility of Fluconazole-Resistant Candida albicans to Thyme Essential Oil. Microorganisms, 9(12), 2454.
Agarwal, A., Xie, B., Vovsha, I., Rambow, O., & Passonneau, R. J. (2011,). Sentiment analysis of twitter data. In Proceedings of the workshop on language in social media (LSM 2011) (pp. 30-38).
Wayne, R. O. (2019). Light and video microscopy. Academic Press.
Tamai, I. A., Pakbin, B., & Fasaei, B. N. (2021). Genetic diversity and antifungal susceptibility of Candida albicans isolates from Iranian HIV-infected patients with oral candidiasis. BMC research notes, 14(1), 1-7.
Kaur, R., Goyal, R.; Dhakad, M. S., Bhalla, P. and Kumar, R. (2014). Epidemiology and Virulence Determinants including Biofilm Profile of Candida Infections in an ICU in a Tertiary Hospital in India,‖ Journal of Mycology, vol. 2014, Article ID 303491, 8 pages, 2014.
Rigo, G. V., & Tasca, T. (2020). Vaginitis: Review on drug resistance. Current Drug Targets, 21(16), 1672-1686.
Hassanpour, P., Hamishehkar, H., Bahari Baroughi, B., Baradaran, B., Sandoghchian Shotorbani, S., Mohammadi, M., ... & Nami, S. (2021). Antifungal Effects of Voriconazole-Loaded Nano-Liposome on Fluconazole-Resistant Clinical Isolates of Candida albicans, Biological Activity and ERG11, CDR1, and CDR2 Gene Expression. ASSAY and Drug Development Technologies, 19(7), 453-462.
Espinel-Ingroff, A., Turnidge, J., Alastruey-Izquierdo, A., Botterel, F., Canton, E., Castro, C.Tejada, M. (2019). Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre YeastOne colorimetric broth and Etest agar diffusion methods. Antimicrobial agents and chemotherapy, 63(1), e01651-18..
Maras, B., Maggiore, A., Mignogna, G., D’Erme, M., & Angiolella, L. (2021). Hyperexpression of CDRs and HWP1 genes negatively impacts on Candida albicans virulence. Plos one, 16(6), e0252555.
Hajar, A. A., Dakhili, M., Saghazadeh, M., Aghaei, S., & Nazari, R. (2022). Changes of Gene Expression in Candida albicans Isolates from Vaginal Infections by Effects of Zinc Oxide Nanoparticles and Fluconazole. Jundishapur Journal of Microbiology, 15(6).
Fan, X.a. ;Wang, Y.; Guo, F.; Zhang, Y. and Jin, T. 2020. Atomicresolution structures of type I ribosome inactivating protein alphamomorcharin with different substrate analogs. International J. of Biolo., Macromolecules, 164: 265–276..
Lu, J.; Zhu, Z.; Zheng, Y. and Shaw, P. (2020). Engineering of Ribosomeinactivating Proteins for Improving Pharmacological Properties, Rev. Toxins, 12:167.
Kumar, R. (2009). Generation of monoclonal antibodies against covalently linked cell wall proteins of Candida albicans (Doctoral dissertation).
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