Identification of Acinetobacter baumannii Isolated from Different Infections and Study the Prevalence of Antibiotic Resistance in Patients of Baquba City
DOI:
https://doi.org/10.24237/ASJ.02.02.712AKeywords:
: Acinetobacter baumannii, Vitik2, Antibiotics ,quinolone, Imipenem,OxidaseAbstract
Acinetobacter baumannii one of the important multidrug-resistant (MDR) opportunistic nosocomial pathogens, in part due to its high capacity of acquiring resistance to diverse antibiotic groups. Twenty five Clinical sample samples were gathered from various sources from Baquba Teaching Hospital, from the period june2022 to October 2022 after consulting with the expert doctor, from both in- and out-patients, and the samples were sent to the lab. Each sample's data was entered onto a unique form, and they included: 10 (sputum samples from respiratory tract infections; 5 burn samples; 4 wound samples; 6 samples for urinary tract infections. After being cultured on MacConkey agar and Blood agar, the isolates were identified. Oxidase and Catalase biochemical tests were performed on the samples, and Vitik2 was employed to make the final diagnosis. Twelve isolates of Acinetobacter baumannii were found after culture and identification; of these, five were found in sputum, two in burns, two in urinary tract infections, and three in wounds. Results of 10 different antibiotics' sensitivity tests on A. baumannii isolates from various clinical sources at the Baquba Teaching Hospital showed that the bacterium showed varying degrees of resistance to the medications.. 100% of the samples displayed resistance to Cefoxitin, Oxacillin, Ceftazidim, Cefepime, and Tetracyclin, while 75% displayed excellent susceptibility to Imipenem, The resistance patterns of isolates were also determined isolates showed to quinolone antibiotic such as Levofloxacin 6/12( 50%), Norfloxacin 7/12 (58.33%) , Ofloxacin 6/12( 50%) ,Ciprofloxacin 3 /12(25%).
References
Z. Moulana, A. Babazadeh, Z. Eslamdost, M. Shokri, S. Ebrahimpour, Phenotypic and genotypic detection of metallo-beta-lactamases in Carbapenem resistant Acinetobacter baumannii, Caspian Journal of Internal Medicine, 11(2),171(2020)
G. J. Da Silva, S. Domingues, Insights on the horizontal gene transfer of carbapenemase determinants in the opportunistic pathogen Acinetobacter baumannii, Microorganisms, 4(3), 29(2016)
A. Fallah, M. A. Rezaee, A. Hasani, M. H. S. Barhaghi, H. S. Kafil, Frequency of bap and cpaA virulence genes in drug resistant clinical isolates of Acinetobacter baumannii and their role in biofilm formation, Iran J Basic Med Sci, 20(8), (2017)
H. Yakkala, D. Samantarrai, M. Gribskov, D. Siddavattam, Comparative genome analysis reveals niche-specific genome expansion in Acinetobacter baumannii strains, PloS one, 14(6), (2019)
S. K.Yadav, R. Bhujel, P. Hamal, S. K. Mishra, S. Sharma, J. B. Sherchand, Burden of Multidrug-Resistant Acinetobacter baumannii Infection in Hospitalized Patients in a Tertiary Care Hospital of Nepal, Infection and Drug Resistance, 13, 725, (2020)
D. Wong, T. B. Nielsen, R. A. Bonomo, P. Pantapalangkoor, B. Luna, B. Spellberg, Clinical and pathophysiological overview of Acinetobacter infections: a century of challenges, Clinical microbiology reviews, 30(1), 409-447(2017)
PA.Wayne, Performance Standards for Antimicrobial Susceptibility Testing, 27th ed., (Clinical and Laboratory Standards Institute, 2019)
L. W. Roberts, B. M. Forde, T. Hurst, W. Ling, G. R. Nimmo, H. Bergh, P. N. Harris, Genomic surveillance, characterisation and intervention of a carbapenem-resistant Acinetobacter baumannii outbreak in critical care, medRxiv., (2020)
CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed., (Wayne, PA: Clinical and Laboratory Standards Institute, 2017)
J. F. MacFaddin, Biochemical Tests for Identification of Medical Bacteria, Williams and Wilkins, Philadelphia, PA,113(2000)
L. Munoz-Price, M. D. MDAR, Acinetobacter Infection, N Engl J Med, 358, 1271-81(2008)
N. Sohrabi, S. Farajnia, M. T. Akhi, M. R. Nahaei, B. Naghili, A. Peymani, Z. Amiri, M. A. Rezaee, N. Saeedi, Prevalence of OXA-type β-lactamases among Acinetobacter baumannii isolates from Northwest of Iran, Microbial drug resistance, 18(4), 385-389(2012)
B. S. Lopes, L. Gallego, S. G. B. Amyes, Multi-drug resistance profiles and the genetic features of Acinetobacter baumannii isolates from Bolivia, The Journal of Infection in Developing Countries, 7(04), 323-328(2013)
F. Akrami, A.E. Namvar, Acinetobacter baumannii as Nosocomial Pathogenic Bacteria, Molecular Genetics, Microbiology and Virology, 34(2), 84-96(2019)
A. O. Lerner, J. Abu-Hanna, Y. Carmeli, V. Schechner, Environmental contamination by carbapenem-resistant Acinetobacter baumannii: The effects of room type and cleaning methods, Infection Control & Hospital Epidemiology, 41(2), 166-171(2020)
G. Di Venanzio, K. H. Moon, B. S. Weber, J. Lopez, P. M. Ly, R. F. Potter, G. Dantas, M. F. Feldman, Multidrug-resistant plasmids repress chromosomally encoded T6SS to enable their dissemination, Proceedings of the National Academy of Sciences, 116(4),1378-1383(2019)
U. E. I. AL Hadeedy, N. B. Mahdi, I. S. AL Jebory, Isolation and Diagnosis of Acinetobacter Bumanniii Recently Isolated From Patients in Kirkuk Hospitals and Study their Antibiotics Resistance, Kirkuk University Journal-Scientific Studies, 14(3), 155-173(2019)
S. M. Kadom, I. N. ABID, Detection of bla OXA-51-like and bla VIM Carbapenemase Genes in Acinetobacter baumannii isolated from burn patients, International Journal of Pharmaceutical Research, 12(2), (2020)
O. A. Forson, E. Ayanka, M. Olu-Taiwo, P. J. Pappoe-Ashong, P. J. Ayeh-Kumi, Bacterial infections in burn wound patients at a tertiary teaching hospital in Accra, Ghana, Annals of burns and fire disasters, 30(2), 116(2017)
M. H. Al-Agamy, A. M. Shibl, M. S. Ali, H. Khubnani, H. H. Radwan, D. M. Livermore, Distribution of β-lactamases in carbapenem-non-susceptible Acinetobacter baumannii in Riyadh, Saudi Arabia, Journal of global antimicrobial resistance, 2(1),17-21(2014)
S. Begum, F. Hasan, S. Hussain, A. A. Shah, Prevalence of multi drug resistant Acinetobacter baumannii in the clinical samples from Tertiary Care Hospital in Islamabad, Pakistan, Pakistan journal of medical sciences, 29(5),1253(2013)
T. Güven, G. Yilmaz, H. R. Güner, A. K. Kalem, F. Eser, M. A. Taşyaran, Increasing resistance of nosocomial Acinetobacter baumannii: are we going to be defeated, Turkish journal of medical sciences, 44(1),73-78(2014)
L. Rahbarnia, S. Farajnia, H. Khaneshi, H. Farajnia, B. Naghili, A. Tanomand, Detection of blaOXA-23 and blaNDM-1 carbapenemase among clinical isolates of A. baumannii in Tabriz, north-west of Iran, Gene Reports, 18,100555(2020)
I. M. S. AL-Kadmy, A. N. M. Ali, I. M. A. Salman, S. S. Khazaal, Molecular characterization of Acinetobacter baumannii isolated from Iraqi hospital environment, New Microbes and New Infections, 21(C), (2018)
R. Maryam, Y. Z. Golnaz, O. Mojgan, T. Malihe, A. Nour, Identification of five phylogenic groups of carbapenemase (blaOXA-23,24,51,58,143) in Acinetobacter baumannii strains isolated from clinical samples in Iran by multiplex PCR, Der Pharma Chem, 7(7), 11-6(2015)
M. D. Huband, R. E. Mendes, M. A. Pfaller, J. M. Lindley, G. J. Strand, V. J. Benn, J. Zhang, L. Li, M. Zhang, X. Tan, Q. Liu, In vitro activity of KBP-7072, a novel third-generation tetracycline, against 531 recent geographically diverse and molecularly characterized Acinetobacter baumannii species complex isolates, Antimicrobial Agents and Chemotherapy, 64(5), (2020)
S. G. I. Subramanian, S. Arora, S. Mehta, R. Mittal, A. Mane, S. N. Charugulla, Susceptibility Pattern of Doxycycline in Comparison to Azithromycin, Cefuroxime and Amoxicillin against Common Isolates: A Retrospective Study Based on Diagnostic Laboratory Data, Journal of The Association of Physicians of India, 68,59, (2020)
K. K. Lahiri, N. S. Mani, S. S. Purai, Acinetobacter spp as nosocomial pathogen: Clinical significance and antimicrobial sensitivity, Medical Journal Armed Forces India, 60(1),7-10(2004)
A. Potron, L. Poirel, P. Nordmann, Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: mechanisms and epidemiology, Int. J. Antimicrob. Agents 45, 568–585(2015)
J. O’Neill, Tackling drug-resistant infections globally: final report and recommendations, in Review on Antimicrobial Resistance, ed. J. O’Neill, (London: Review on Antimicrobial Resistance, 2016)
C. A. Nsofor, V. N. Nwokenkwo, C. U. Ohale, Prevalence and antibiotic susceptibility pattern of Staphylococcus aureus isolated from various clinical specimens in South East Nigeria, MOJ Cell Sci Rep, 3(2),1-5(2016)
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