Activity Of  Ciprofloxacin and  Resveratrol  On Expression Of  AdeI J Genes In Clinical Acinetobacter baumannii Isolates : Activity Of  Ciprofloxacin and  Resveratrol  On Expression Of  AdeI J Genes In Clinical Acinetobacter baumannii Isolates

Muntaha A.  Abdulkareem; kareem Ibrahim Mubarak

doi:10.24237/ASJ.02.03.793B

Authors

Muntaha A. Abdulkareem Department of Biology, College of Science, University of Diyala, Iraq
kareem Ibrahim Mubarak Department of Biology, College of Science, University of Diyala, Iraq

DOI:

https://doi.org/10.24237/ASJ.02.03.793B

Keywords:

A . baumannii, AdeI J genes , Antibiotic , Efflux pump inhibitors

Abstract

baumannii considered one important pathogenic bacteria and responsible for several nosocomial and community infections for human and , possess the ability to form biofilms , and continued development of antibiotic resistance to drugs used to treat bacterial infections . Current study was performed ,during the period from 1/9 /2022 to the end of December 30 /12 /2022 ,a total of 210 of blood specimens were collected in Baqubah Teaching Hospital /Iraq to investigate the prevalence of bacterial isolates among patients in Intensive Care Unit (ICU) , detect antibiotic resistance , biofilms formation , molecular detection of efflux pumps adeIJ genes ,and activity of ciprofloxacin and resveratrol on expression of this two genes. The results revealed that that the percentage of positive growth of A. baumannii which isolates from blood samples with among ICU patients were 12 % , presence of highest resistance of 25 isolates against fourteen types of antibiotics, our finding showed significant differences before and after treatment of resistance isolates with Resveratrol with a higher reduction expression of AdeIJ genes in all isolates under study were reach 0.59 and 0.28 respectively.

Author Biography

kareem Ibrahim Mubarak, Department of Biology, College of Science, University of Diyala, Iraq

References

I. Chapartegui-Gonzalez, M. Lazaro-Diez, Z. Bravo, J. Navas, J. M. Icardo, J. Ramos-Vivas, Acinetobacter baumannii maintains its virulence after long-time starvation, Public Library of Science One, 13(8), e0201961(2018)

M. Nguyen, S .G. Joshi, Carbapenem Resistance in Acinetobacter baumannii, and their Importance in Hospital‐acquired Infections: A Scientific Review, Journal of Applied Microbiology, 131(6), 2715–2738(2021)

A. Z. Kanafani, S. S. Kanj, Ministry of Health, Kingdome of Saudi Arabia, (2014)

S. B. Al-masaudi, Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features, Saudi journal of biological sciences, 25(3),586-596(2018)

N. Guo, W. Xue, D. Tang, J. Ding, B. Zhao, Risk factors and outcomes of hospitalized patients with blood infections caused by multi drug resistant Acinetobacter baumannii complex in a hospital of Northern China, Am J Infect Control, 44(4),e37-9(2016)

M. N. L. Kurihara, R. O. de Sales, K. E. da Silva, W. G. Maciel, S. Simionatto , Multidrug-resistant Acinetobacter baumannii outbreaks: a global problem in healthcare settings, Journal of the Brazilian Society of Tropical Medicine, 53,(e20200248), (2020)

J. Melnick, Adelbergs, Medical Microbiology, 28th .ed., (The McGraw-Hill Companies , Inc., New York, 2019), 258 -259

R. Kumar, S. Pooja Patial, A Review on Efflux Pump Inhibitors of Gram-Positive and Gram-Negative Bacteria from Plant Sources, Current Microbiology and Applied Sciences, 5, 837-855(2016)

M. J. Cheesman, A. Ilanko, B. Blonk, I. E. Cock, Developing New Antimicrobial Therapies: Are Synergistic Combinations of Plant Extracts/Compounds with Conventional Antibiotics the Solution, Pharmacognosy Reviews , 11, 57(2017)

L. Kuršvietienė, I. Stanevičienė, A. Mongirdienė, J. Bernatonienė, Multiplicity of effects and health benefits of resveratrol, Medicina, 52(3), 148-155(2016)

A. Duarte, A .C. Alves, S. Ferreira, F. Silva, F .C. Domingues, Resveratrol inclusion complexes: antibacterial and anti-biofilm activity against Campylobacter spp. and Arcobacter butzleri, Food Research International, 77, 244-250(2015)

S. Ferreira, F. Domingues, The antimicrobial action of resveratrol against Listeria monocytogenes in food‐based models and its antibiofilm properties, Journal of the Science of Food and Agriculture, 96(13), 4531-4535(2016)

M .V. Selma, M. Larrosa, D. Beltrán, R. Lucas, J .C. Morales, F. Tomás-Barberán, J. C. Espín, Resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives reduce Escherichia coli O157: H7, Salmonella Typhimurium, and Listeria monocytogenes Scott A adhesion to colonic epithelial cell lines, Journal of agricultural and food chemistry, 60(30), 7367-7374(2012)

S. Rovelli, The role of polysaccharides in plasmodium falciparum malaria, (2010)

S. Constantiniu, A. Romaniuc, L .S. Iancu, R. Filimon, I. Taraşi, Cultural and biochemical characteristics of Acinetobacter spp. strains isolated from hospital units, The journal of preventive medicine, 12(3-4), 35-42(2004)

A. O. Darweesh, A. A. Shareef, F. A. A. Alriyahee, Isolation and Identification of Cellulytic Ruminant Pseudomonas Aeruginosa by Classical and Molecular Methods in Basrah Province, Annals of the Romanian Society for Cell Biology, 4672-80(2021)

T. Addis, Y. Mekonnen, Z. Ayenew, S. Fentaw, H. Biazin, Bacterial Uropathogens And Burden Of Antimicrobial Resistance Pattern In Urine Specimens Referred To Ethiopian Public Health Institute, Plos One, 16, E0259602(2021)

B. Trupti, G. S. Bhatambare, M. Varma, M. Pandey, Species-level identification of Acinetobacter by 16s rRNA sequencing: Necessity today, essentiality tomorrow, New Nigerian Journal of Clinical Research, 5, 55, (2016)

J .G. Cappuccino, C .T. Welsh, Microbiology: A Laboratory Manual, 11th ed (Pearson Education, England, 2018)

CLSI, Performance Standards for Antimicrobial Susceptibility Testing, 27th ed (Wayne, PA: Clinical and Laboratory Standards Institute, 2022)

A. Louie, D. L. Brown, W. Liu, R.W. Kulawy, M. R. Deziel, G. L. Drusano, In vitro infection model characterizing the effect of efflux pump inhibition on prevention of resistance to levofloxacin and ciprofloxacin in Streptococcus pneumoniae, Antimicrobial agents and chemotherapy, 51(11), 3988-4000(2007)

L. Ghellai, H. Hassaine, N. Klouche, A. Khadir, N. Aissaoui, F. Nas, W. ZINGG, Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus isolated in Algeria at the University, African Journal of Bacteriology Research, 6(1), 1-6(2014)

L. Lin, B .D. Ling, X. Z. Li, Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii–Acinetobacter calcoaceticus complex, International journal of antimicrobial agents, 33(1), 27-32(2009)

L. J. Kenneth, D. T. Schmittgen Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method, methods, 25, 402-08(2001)

T. A. N. V. I. R. Kaur, C. H. A. Y. A. N. I. K. A. Putatunda, A. R. O. M. A. Oberoi, A. S. H. I. S. H. Vyas, G. A. U. R. A. V. Kumar, Prevalence and drug resistance in Acinetobacter sp., isolated from intensive care units patients in Punjab, India, Asian J Pharm Clin Res, 11(14), 88-93(2018)

H.Benjamin, B. Reyneke, T. Ndlovu, W. Khan, Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains, Microbial pathogenesis, 172, 105749(2022)

A. M. I. R. Peymani, S. A. F. A. R. FARAJnIA, M. R. Nahaei, N. Sohrabi, L. A. L. E. H. Abbasi, K. H. A. L. I. L. Ansarin, F. A. T. E. M. E. H. Azhari, Prevalence of class 1 integron among multidrug-resistant Acinetobacter baumannii in Tabriz, northwest of Iran, Pol J Microbiol, 61(1),57-60(2012)

A. Muhammad, I. A. Alvi, S. U. Rehman, Insight into Acinetobacter baumannii: pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities, Infection and drug resistance, 1249-60(2018)

J. F. Turton, M. E. Ward, N. Woodford, M. E. Kaufmann, R. Pike, D. M. Livermore, T. L. Pitt, The role of IS Aba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii, FEMS microbiology letters, 258(1), 72-77(2006)

H. Kadhom, M. Ali, Epidemiological molecular analysis of Acinetobacter baumannii isolates using a multilocus sequencing typing and global lineage, Revis Bionatura, 7, 29(2022)

S. M. M. AL-Dahlaki, Molecular detection and gene expression for hcp and blaOXA-51 genes in Acinetobacter baumannii isolated from different clinical sources, MSC Thesis, College of Science, University of Diyala, Diyala, Iraq, 154(2020)

M. Jassim, K. I. Mubarak, K. D. Wadi, Study of Antibacterial Activity from Alcoholic Extracts of Torilis arvensis (Huds.) Link on Biofilm formation of Acinetobacter baumannii in Diyala Province-Iraq, Academic Science Journal, 1, 60-76(2023)

S. M. Kadom, N. A. intidhaar, Detection of bla OXA-51-like and bla VIM Carbapenemase Genes in Acinetobacter baumannii isolated from burn patients, International Journal of Pharmaceutical Research, 12, (2020)

S. Swaminathan, G. Immanuel, S. Arora, S. Mehta, R. Mittal, A. Mane, S. N. Charugulla, Susceptibility pattern of doxycycline in comparison to azithromycin, cefuroxime and amoxicillin against common isolates: a retrospective study based on diagnostic laboratory data, Journal of The Association of Physicians of India, 68, 59(2020)

U. E. I. AL Hadeedy, N. B. Mahdi, I. S. AL Jebory, Isolation and Diagnosis of Acinetobacter bumanniii Recently Isolated From Patients in Kirkuk Hospitals and Study their Antibiotics Resistance, Kirkuk University Journal-Scientific Studies, 14, 155-73(2019)

M. A. J. F. M. L. O’conor, D. K. Cadet USMA, C. B. Joseph, Evaluation of Virulence Gene Expression Patterns in Acinetobacter baumannii Using Quantitative Real-Time Polymerase Chain Reaction Array, MILITARY MEDICINE, 181, (2017)

M. TH. S. AL-Dulaimi, Antimicrobial activity of Colistin combined with some Probiotics against the clinical isolates of Acinetobacter baumannii bacteria, MSC. Thesis, College of Science, University of Diyala, (2021)

L. Liu, X. Shen, J. Yu, X. Cao, Q. Zhan, Y. Guo, F. Yu, Subinhibitory concentrations of fusidic acid may reduce the virulence of S .aureus by down-regulating sara and saers to reduce biofilm formation and α-toxin expression, Frontiers in Microbiology, 11,25 ,(2020)

U. Singkham-In, P. G. Higgins, D. L. Wannigama, P. Hongsing, T. Chatsuwan,.Rescued chlorhexidine activity by resveratrol against carbapenem-resistant Acinetobacter baumannii via down-regulation of AdeB efflux pump, PLoS One, 15(12), e0243082(2020)

M. R. Shakibaie, In Silico Characterization Of Biofilm-Associated Protein (Bap) Identified In A Multi-Drug Resistant A .baumannii Clinical Isolate, Journal Of Medical Microbiology And Infectious Diseases, 9, 210- 220(2021)

M. A. Narjis, M. S. Mahdi, Isolation and identification of multi-drug resistance Acinetobacter baumannii isolated from clinical samples at Baghdad, Iraq, Journal of Applied and Natural Science, 15(2), 663-671(2023)

S. Wagner, R. Sommer, S. Hinsberger, C. Lu, R. Hartmann, W. Empting, M. Titz, Novel strategies for the treatment of Pseudomonas aeruginosa infections, J. Med .Chem, 59, 5929 − 5969(2016)

E. F. Haney, M. J. Trimble, R. E. Hancock, Microtiter Plate Assays To Assess Antibiofilm Activity Against Bacteria, Nature Protocols, 16, 2615-2632(2021)

D. S. Auld, P. A. Coassin, N. P. Coussens, P. Hensley, C. Klumpp-Thomas, S. Michael, G. S. Sittampalam, O. J. Trask, B. K. Wagner, J. R. Weidner, Microplate Selection And Recommended Practices In High-Throughput Screening And Quantitative Biology, Assay Guidance Manual, (2020)

Y. Feng, S. Zou, H. Chen, Y. Yu, Z. Ruan, Bacwgstdb 2.0: A One-Stop Repository For Bacterial Whole-Genome Sequence Typing And Source Tracking, Nucleic Acids Research, 49, D644-D650(2021)

W. Gamal, C. Brandt, L. D. Sprague, H. Neubauer, M. W. Pletz, WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective, BMC microbiology, 21, 1-13(2021)

M. A. Ali, S. S. Mahmood, Role of RND-efflux pump in levofloxacin resistance among Acinetobacter baumannii, Iraqi Journal of Agricultural Sciences, 53, 542-50(2022)

C. F. Xu, S. R. Bilya, W. Xu, adeABC efflux gene in Acinetobacter baumannii, New microbes and new infections, 30, 100549(2019)

B. Choi, M. C. Choudhary, J. Regan, J. A. Sparks, R. F. Padera, X. Qiu, J. Z. Li, Persistence and evolution of SARS-CoV-2 in an immunocompromised host, New England Journal of Medicine, 383(23), 2291-2293(2020)

L. Li, J. Yu, X. Shen, X. Cao, Q. Zhan, Y. Guo, F. Yu, Resveratrol enhances the antimicrobial effect of polymyxin B on Klebsiella pneumoniae and Escherichia coli isolates with polymyxin B resistance, BMC microbiology, 20, 1-8(2020)

C. B. Landersdorfer, N. S. Ly, H. Xu, B.T. Tsuji, J. B. Bulitta, Quantifying subpopulation synergy for antibiotic combinations via mechanism-based modeling and a sequential dosing design, Antimicrobial agents and chemotherapy, 57(5), 2343-2351(2013)

E. J. Yoon, P. Courvalin, C. Grillot-Courvalin, RND-type efflux pumps in multidrug-resistant clinical isolates of Acinetobacter baumannii: major role for AdeABC overexpression and AdeRS mutations, Antimicrobial agents and chemotherapy, 57(7), 2989–2995(2013)

A. J. Seukep, H. G. Mbuntcha, V. Kuete, Y. Chu, E. Fan, M. Q. Guo, What Approaches to Thwart Bacterial Efflux Pumps-Mediated Resistance, Antibiotics, 11(10), 1287(2022)

A. Duartea, C. Ana Alves AC, S. Ferreira, F. Silva, FC. Dominguesa, Resveratrol inclusion complexes: Antibacterial and anti-biofilm activity against Campylobacter spp. and Arcobacter butzleri, Food Res Int, 77(2),244 –250(2015)

L. Kursvietiene, I. Staneviciene, A. Mongirdiene, J. Bernatoniene, Multiplicity of effects and health benefits of resveratrol, Medicina (Kaunas), 52,148–155(2016)

P. Verma, M. Tiwari, V. Tiwari, Strategies to combat bacterial antimicrobial resistance: a focus on mechanism of the efflux pumps inhibitors, SN Comprehensive Clinical Medicine, 3, 510-527(2021)

Activity Of Ciprofloxacin and Resveratrol On Expression Of AdeI J Genes In Clinical Acinetobacter baumannii Isolates