Study of Antibacterial Activity from Alcoholic Extracts of Torilis arvensis (Huds.) Link on Biofilm formation of Acinetobacter baumannii in Diyala Province - Iraq

Abstract

This study was conducted in Baqubah Education Hospital in Diyala/Iraq from the period 1^st January to 2^nd of March 2022. One hundred twenty clinical specimens from wounds and burns swab of different ages and gender and were obtained and cultured on suitable media (Blood agar and MacConkey agar) for isolation and identification of Acinetobacter baumannii according to standard bacteriological techniques incubated under suitable conditions. The current study was investigated for antibiotic susceptibility test, Minimum, Sub Inhibitory concentration (MIC and SUB-MIC) of antibiotics, biofilms formation, antibacterial effect of Methanol and ethanol extracts of Torilis arvensis (Huds.) link and active compounds of extract. Under current study, twenty isolates of A.baumannii were identified and which were distributed as (65 %) from wounds infections and 35% from burns infections. The Minimum Inhibitory Concentration of Meropenem and Levofloxacin ranged from 8-512 ug.m^-l. Antibiotics sensitivity test of bacteria revealed highly resistance for all antibiotics which were used in current study where the percentage value range from 46% for Tobramycin to 90% for Ampicillin-sulbactam and Ceftazidime, Isolates of A.baumannii showed high ability for biofilm formation with a ratio about 85 %. And 20 substances that were present in plant extract were detected by Gas Chromatography-mass spectrometry and recorded as active compounds. Ethanol and methanol extracts of Torilis arvensis (Huds.) Link demonstrated high antibacterial activity against A. baumannii isolates as concentration about 200 mg.ml^-1. The results of the current study showed that the stimulation of A.baumannii isolates for biofilm formation may be affected by the both SUB-MIC of antibiotics and Torilis arvensis (Huds.) Link.