Investigation of in vitro Anti Protoscoleces activity of Cyperus rotundus plant extract
DOI:
https://doi.org/10.24237/Keywords:
Echinococcus granulosus, Protoscoleces , Cyperus rotundusAbstract
Hydatid cyst is a continuing health problem in various parts of the world that is not linked to the economic level of countries, but rather linked to adherence to health conditions. Therefore, both animals and humans are still at risk of infection. The study was conducted to evaluate the effect of the boiled water extract of Cyperus rotundus on the viability of Echinococcus granulosus protoscoleces in vitro as an environmentally friendly means of controlling the disease.
Livers were collected from the slaughtered animals at the butchers’ shops )cows and sheep) infected with hydatid cysts from different areas, including butchers’ Baladruz and Baquba in Diyala province. The concentrated suspension of protoscoleces was added to different concentrations of the boiled water extract of Cyperus rotundus root at different concentrations (100, 50, 25, 12.5 mg/ml), and for different time periods (5, 4, 3, 2, 1, 30 hours), and the viability of these protoscoleces was measured using 0.1% aqueous eosin stain.
The results of the current study showed that the mortality rate of protoscoleces was associated with an increase in the concentration of the plant extract and the exposure time. The highest mortality rate (100%) was at the highest concentration of 100 mg/ml within 240 minutes, while the lowest mortality rate (34.54%) was at the concentration of 12.5 mg/ml and within half an hour (30 minutes). The differences in mortality rates of protoscoleces at different concentrations and different exposure times were significant at (p<0.05).In conclusion, The extract has a potential therapeutic ability and can be used to treat and treat cysts.
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