Detection of Some Virulence Factors and aac(6)Ib Resistance Gene in Clinical Proteus mirabilis isolates

Abstract

The Proteus mirabilis. are opportunistic members of Enterobacteriaceae ,accounting for 10% of urinary tract infections and other primary clinical infections. They produce beta-lactamases that can confer resistance to beta-lactam antibiotics. This study aimed to investigate the prevalence of some virulence factors with antimicrobial resistance and molecular characteristics of  aac-Ib gene The current study have been included 250 specimens were collected from urine sample, wound, burn, diabetic foot, and vaginal swabs conducted in Baquba Teaching Hospital, Al-Batool Teaching Hospital, and AlKhalis hospital in Diyala province during the period extended from August 2023 to January 2024. A total of 250 specimens Isolates were identified by morphological form on blood agar and macConkey agar, traditional biochemical tests. The results showed that 75 isolates were identified as P. mirabilis (30%) consisted of 35 isolates (46.7%) obtained from urine, 8 isolates (10.7%) obtained from wound, 10 isolates (13.3%) obtained from burns and diabetic foot and 12 isolates (16%) obtained from vaginal swabs. The higher rate of positive isolates were recorded in urine samples. Females isolation rate was much more than in males 60% and 40% respectively and patients age group 21- 30 year was more prevalence than other groups. It was found that the more effective antibiotic against isolates was the norfloxacin followed by levofloxacin, where all isolates were sensitive to it. On the other hand, the less effective antibiotics were amikacin and oxacillin when they were resisted by all isolates.