Biofilm Formation by Protues Mirabilis Isolated From Patients Suffering From Urinary Tract Infections in Baqubah City
DOI:
https://doi.org/10.24237/ASJ.02.04.865AKeywords:
Biofilm, Vancomysin, Absorbency value, ResistanceAbstract
Protues mirabilis has the ability to induce urinary tract infections primarily has to do with the
formation of biofilms on catheter and UTI surfaces. This bacteria expresses a number of
virulence factors essential to the creation of biofilms. Fifty urine specimens were taken from
patients who were hospitalized and under treatment from the clinical laboratories of Baquba
Teaching Hospital. Of those fifty specimens, fifteen clinical specimens were positive for
isolates of Protues mirabilis. The results showed that all the P. mirabilis isolates under study
were 100% resistant to the Gentamicin, Cefotaxime and the resistance of the isolates to
Vancomysin and Azithromycin were 90%, Cefixime, Augmentin by 80%, and their resistance
to Piperacillin-tazobactam by 70%. According to these percentages, the isolates were divided
into two classes Extensively Drug Resistance (XDR) (90%), which were resistant to the seven
antibiotics that were used in the study, and (10%) were Multi Drug Resistance (MDR) which
were resistant to four antibiotic. Results showed that the P. mirabilis have the ability to produce
biofilm, all isolates could produce biofilm but in deferent percentage 70% were strong ,30%
were moderate, and the absorbency value was ranged from (0.353 – 0.159). The convention
PCR analysis revealed that the gene (Esp), encoding the enzymes that cause Proteus mirabilis
to form biofilm, accounted for 15 (40%) of the total.
References
R. Mann, D. G. Mediati, I. G. Duggin, E. J. Harry, A. L. Botomley, Metabolic
adaptations of uropathogenic E. coli in the urinary tract. Frontiers in cellular and
infection microbiology, 7, 241(2017(
A. L. Flores-Mireles, J. N. Walker, M. Caparon, S. J. Hultgren, Urinary tract
infections: epidemiology, mechanisms of infection and treatment options, Nature
reviews microbiology, 13, 269-284(2015)
R. Wasfi, S. M. Hamed, M. A. Amer, L. I. Fahmy, Proteus mirabilis biofilm:
development and therapeutic strategies, Frontiers in cellular and infection
microbiology, 10, 414(2020)
M. Gajdacs, E. Urban, Comparative epidemiology and resistance trends of proteae in
urinary tract infections of inpatients and outpatients: A 10-year retrospective study,
Antibiotics, 8, 91)2019(
E. J. Giamarellos-Bourboulis, E. Papadimitriou, N. Galanakis, A. Antonopoulou, T.
Tsaganos, K. Kanellakopoulou, H. Giamarellou, Multidrug resistance to antimicrobials
as a predominant factor influencing patient survival, International journal of
antimicrobial agents, 27, 476-481)2006(
R. Baldiris, V. Teheran, R. Vivas-Reyes, A. Montes, O. Arzuza, (2016)
M. P. Weinstein, J. S. Lewis, Clinical and Laboratory Standards Institute (CLSI).
Performance Standards for Antimicrobial Susceptibility Testing, 30th ed. CLSI
supplement M100 ,(Clinical and Laboratory Standards Institute, 950West Valley
Road, Suite 2500, Wayne, Pennsylvania 19087 USA:47), (2020)
L. Ghellai, H. Hassaine, N. Klouche, A. Khadir, N. Aissaoui, F. Nas, ZINGG W
Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus
isolated in Algeria at the University, Afr. J. Bacteriol. Res., 6(1), 1-6(2014)
S. Bose, M. Khodke, S. Basak, S. K. Mallick, Detection of biofilm producing
staphylococci need of the hour, J. Clin. And Diagnostic Res., 3(6), 1915-1920(2009)
L. S. Munoz-Price, R. A. Weinstein, Acinetobacter infection, New England Journal of
Medicine, 358(12), 1271-1281(2008)
G. A. O'Toole, Microtiter dish biofilm formation assay, J. Vis. Exp., 47, 2437(2011)
A. Mirzaei, M. Habibi, S. Bouzari, M. R. Karam, Characterization of Antibiotic-
Susceptibility Patterns, Virulence Factor Profiles and Clonal Relatedness in Proteus mirabilis Isolates from Patients with Urinary Tract Infection in Iran, Infect. Drug.
Resist., 12,3967-79(2019)
R. A. Sayal, N. M. Alkharasani, A. A. Alsadawi, Z. H. O. Alquraishi, Molecular study
of biofilm and some antibiotic resistance gene in Proteus mirabilis isolated from
children with UTI patients in Al-najaf Governorate, Journal of Pharmaceutical
Sciences and Research, 10(8), 1986-1990(2018)
S. Á. Jacobsen, D. J. Stickler, H. L. T. Mobley, M. E. Shirtliff, Complicated catheterassociated
urinary tract infections due to Escherichia coli and Proteus mirabilis,
Clinical microbiology reviews, 21(1), 26-59(2008)
S. M. Jacobsen, M. E. Shirtliff, Proteus mirabilis biofilms and catheter-associated
urinary tract infections, Virulence, 2, 460-465)2011(
I. Keren, N. Kaldalu, A. Spoering, Y. Wang, K. Lewis, Persister cells and tolerance to
antimicrobials, FEMS microbiology letters, 230(1),13-18(2004)
G. D. I. De Silva, M. Kantzanou, A. Justice, R. C. Massey, A. R. Wilkinson, N. P. J.
Day, S. J. Peacock, The ica operon and biofilm production in coagulase-negative
staphylococci associated with carriage and disease in a neonatal intensive care unit,
Journal of clinical microbiology, 40(2), 382-388(2002)
O. May, N. Merle, R. Paule, C. Noel, L. Roumenina, V. Fremeaux-Bacchi, M. Frimat,
Analyse des propriétés de protection contre l’activation du complément et de
l’expression d’un phénotype prothrombotique de l’endothélium glomérulaire rénal.
Néphrologie & Thérapeutique, 10(5), 272(2014)
N. Høiby, T. Bjarnsholt, M. Givskov, S. Molin, O. Ciofu, Antibiotic resistance of
bacterial biofilms, International journal of antimicrobial agents, 35(4), 322-332(2010)
K.C. Reiter, T. G. Da Silva Paim, C. F. De Oliveira, P. A. D’azevedo, High biofilm
production by invasive multiresistant staphylococci, Apmis, 119(11), 776-781(2011)
F. A. Allawi, Z. Y. Motaweq, Phenotypic and Molecular Correlation Between Biofilm
Production and Antibiotic Resistance of Proteus Mirabilis Isolated From Different
Clinical Sources/Iraq, Turkish Journal of Physiotherapy and Rehabilitation, 32(3),
(2021)
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