Effect of Crude Alkaloid Extract of Cladophora glomerata (L.) kutz. OnGene Expression and Cell Death
DOI:
https://doi.org/10.24237/Keywords:
Cladophora glomerata, cell lines, apoptosis genes, and heat shock proteins genesAbstract
The objective of this study was to determine the physiological effects of the crude alkaloid
extract from Cladophora glomerata at the half inhibitory concentration (300, 100, and 1000)
μg /ml on the growth rate in the gene expression of the heat shock proteins (Hsp90, Hsp70,
Hsp60), as well as the cell death genes (Caspase-8, P53, and Bcl2) of the cancer cell lines
Hepatocellular carcinoma (HepG2), human cervical cancer (Hela), and mice embryo fibroblast
(MEF). In addition to monitoring morphological changes using real-time polymerase chain
reaction for gene analysis using Kapa syber green dye, and observing morphological changes
using Acridine Orange / Propidium Iodid dye that examined using a fluorescence optical
microscope. The results of this study showed that treating cancer cell lines with the crude
alkaloid extract at half inhibitory concentration effectively suppresses the gene expression of
heat shock protein that encode genes and increases the expression of programmed cell death
genes. We conclude that Cladophora glomerata extract plays a major role in inhibiting cancer
cells (HepG2 and Hela cell lines) through regulating heat shock protein genes (Hsp90, HSp70,
HSp60) and apoptosis genes (P53, Bcl2, and Caspase- 8) which have a major role in cancer.
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