Molecular investigation of the presence of (pslD, pslA, rhlR) genes in Pseudomonas aeruginosa isolated from burns and wounds
DOI:
https://doi.org/10.24237/Keywords:
Pseudomonas aeruginosa, Virulence factors, Multi-drug resistance, VITEK2 compact system, Sequence analysis.Abstract
Background: Pseudomonas aeruginosa is one of the most common Gram-negative bacteria responsible for outbreaks of infection and morbidity in hospitals. The difficulty in treating them lies in their possession of a wide range of virulence factors in addition to their ability to have natural and acquired resistance to antibiotics especially the multi-drug resistance (MDR) ones. Material and Methods: 114 samples were collected and isolated from burns and wounds patients hospitalized in Baquba Teaching Hospital, during the period from September to December 2022. These isolates were diagnosed using conventional diagnostic methods, microscope diagnosis, and biochemical tests, and then the final diagnosis was made using the VITEK2 compact system device. Results: 43 isolates 42% were obtained from the total collation samples of P. aeruginosa. A test was conducted to detect the ability to from biofilms, and the results were as follows: (Strong adherent 41%, n=7), (Moderately adherent 23%, n=4), (Weakly adherent 35%, n=6), and they were tested for sensitivity towards 12 antibiotics, and the results were as follows: AMC 96%; CAZ 100%; CTX 100%; CRO 100%; FEP 75%; MEM 76%; AK 82%; TOB 76%; GN 70%; E 94%; PB 23%; CIP 47%. 17 of them were isolates with multidrug resistance (MDR). Sequence analysis was used to detect the presence of (pslD, pslA, rhlR) genes in 17 isolates of P. aeruginosa, and the obtained results were as follows: the presence of the pslD gene at arte of (94%; n=16), the pslA gene at rate of (100%; n=17), and the rhlR gene at rate of (100%; n=17).
References
Abdulzahra, Athraa Najm (2020). Study Multidrug Resistance Factors of Pseudomonas aeruginosa isolated from burns and wounds by phenotypic and molecular methods. Master Thesis, College of Education for Pure Sciences (Ibn Al- Haytham), University of Baghdad.
Abebe, G. M. (2020). The role of bacterial biofilm in antibiotic resistance and food contamination. International journal of microbiology.
Abootaleb, M; Zolfaghari, M, R; Soleimani, N, A; Ghorbanmehr, N; Yazdian, M, R. (2020). Biofilm formation with Microtiter plate 96 and pslA detection of Pseudomonas aeruginosa isolates from clinical samples in Iran. Int. J. Adv. Biol. Bio. Res. 8:58-66.
Al -Azzawi, Shahla Najm Abed (2018). Molecular study of Pseudomonas aeruginosa resistance that isolated from Wounds and burns treated with antiseptics. Master Thesis, College of Education for Pure Sciences (Ibn Al- Haytham), University of Baghdad.
Al-Janabi, Thooalnoon Younes Saleh. (2019). Biosynthesis of Zinc Oxide Nanoparticles by Pseudomonas aeruginosa Locally Isolated. Master Thesis, College of Education for Pure Sciences (Ibn Al- Haytham) University of Baghdad.
Al-kabi, Nawal Hadi Mohsen (2022). Study of sensitivity of Pseudomonas aeruginosa bacteria isolated from burn injuries to some plant extracts and their ability to transmission through insect in Karbala city. Master Thesis, College of Education for Pure Sciences, University of Karbala.
Al-kazrage, Hussein Ali Dawood. (2021). Inhibition of Virulence Factors in Pseudomonas aeruginosa Isolated from Clinical Samples Using Galardin Loaded AgPEG Nanocomposite. Al-Nahrain University, College of Biotechnology.
Al-Shamary, Maha Mikhlef Kadum (2018). The relationship between biofilm formation and pyocyanin producing genes in Pseudomonas aeruginosa isolated from clinical sources. Doctor of Philosophy (Ph.D.) in Biology/ Microbiology, College of Science, Mustansiriyah University.
AL-Sheikhly, M, A, R, H; Musleh, L, N; Al-Mathkhury, H, J, F. (2020). Gene Expression of pelA and pslA in Pseudomonas aeruginosa under Gentamicin Stress. Ira. J. Sci. 61(2): 295-305.
Anjum, H; Arefin, M.S; Jahan, N; Oishee, M.J; Nahar, S; Islam, S; Banerjee, S; Sinha, S; Kumar, S; Haque, M; Rahman, M.H. (2023). Roles of intrinsic and acquired resistance determinants in multidrug-resistant clinical Pseudomonas aeruginosa in Bangladesh. Bangladesh Journal of Medical Science. (22): 489-507.
Asadpour, L. (2018). Antimicrobial resistance, biofilm-forming ability and virulence potential of Pseudomonas aeruginosa isolated from burn patients in northern Iran. J Glob Antimicrob Resist. 13:214-20.
Bogiel, T; Depka, D; Rzepka, M; Mikucka, A. (2022). Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections. Int. J. Mol. Sci. 23(9208): 1-13.
Daigle, D, M; Cao, L; Fraud, S; Wilke, M, S; Pacey, A; Klinoski, R; Strynadka, N, C; Dean, C, R; Poole, K. (2007). Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa. J. Bacteriol. 189, 5441–5451.
El-sayed, H.A; Fahmy, Y. A. (2021). Correlation between biofilm formation and multidrug resistance in clinical isolates of Pseudomonas aeruginosa. Microbes and Infectious Diseases. 2(3): 541-549.
Emami, S; Nikokar, I; Ghasemi, Y; Ebrahimpour, M; Saraie, H, S, E; Araghian, A; Faezi, S; Farahbakhsh, M; Rajabi, A. (2015). Antibiotic Resistance Pattern and Distribution of pslA Gene Among Biofilm Producing Pseudomonas aeruginosa Isolated from Waste Water of a Burn Center. Jun. J. Mic. 8(11): e23669.
Emaneini, M., Kalantar-Neyestanaki, D., Jabalameli, L., Hashemi, M., Beigverdi, R., & Jabalameli, F. (2019). Molecular analysis and antimicrobial resistance pattern of distinct strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Iran. Iranian journal of microbiology, 11(2), 98.
Garallah, E, T. (2015). Molecular analysis of some virulence genes of Pseudomonas aeruginosa isolated from cystic fibrosis and non-cystic fibrosis sources. M.Sc. Thesis. College of Science. AL- Mustansiryia University.
Ghadaksaz, A; Fooladi A, A, I; Hosseini, H, M; Amin, M. (2015). The prevalence of some Pseudomonas virulence genes related to biofilm formation and alginate production among clinical isolates. J. App. Bio. 13:61-68.
Gill, J, S; Arora, S; Khanna, S, P; Kumar, K, H. (2016). Prevalence of multidrug-resistant, extensively drug-resistant, and pan drug-resistant P. aeruginosa from a tertiary level intensive care unit. Journal of global infectious diseases. 8(4):155.
Hameed, Zainab Mohammed. (2017). Detection of CTX-M-1 and CTX-M-3 genes in virulent, Multidrug resistant Pseudomonas aeruginosa isolates. Master Thesis, College of Science, University of Diyala.
Hassuna, N, A; Mandour, S, A; Mohamed, E, S. (2020). Virulence Constitution of Multi-Drug- Resistant Pseudomonas aeruginosain Upper Egypt. Infect Drug Resist. 13:587.
Jaddoa, S, A; Al-Hassani, M, K, A. (2022). Phenotypic and molecular detection of biofilm production by P. aeruginosa isolated from different clinical cases. Int. J. Hea. Sci. 6(S8):5173–5179.
Jalil, M. B.; Abdul-Hussein, Z. R. and AL-Hmudi, H. A. (2017). Isolation and identification of multi drug resistant biofilm prpducer with Burn Wound infection in Basra Provincel/ Iraq IJDR 7:11 pp.
Kireçci, E. & Kareem R.D. (2014) Antibiotic susceptibility patterns of Pseudomonas aeruginosa strains isolated from various clinical specimens Sky Journal of Microbiology Research. 2(2): 013 – 017.
Mahmood, Suha hameed. (2022). Molecular Study of Quorum Sensing Genes in Pseudomonas aeruginosa Isolated from Patients. Master Thesis, College Education for pure Science, University of Diyala.
Mohamed, E, A; Nawar, A, E; Hegazy, E, E. (2023). Insight into quorum sensing genes lasr and rhlr, their related virulence factors and antibiotic resistance pattern in Pseudomonas aeruginosa isolated from ocular infections. Mic. Inf. Dis. 4(2), 575-589.
Mohammed, Enas Ammar (2021). Detection of the Type III secretion system genes in Pseudomonas aeruginosa isolated from clinical sources in Diyala governorate. Master Thesis, College of Education for Pure Science, University of Diyala.
Nasrin, S; Hegerle, N; Sen, S; Nkeze, J; Sen, S; Permala-Booth J, et al. (2022). Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection. BMC microbiology. 22(1):1-2.
Nasser, M.; Ogali, M. and Kharat, A.S. (2018). Prevalence of MDR Pseudomonas aeruginosa of war-related wound and burn ward infections from some conflict areas of Western Yemen. Wound medicine, 20, 58-61.
Ozen, A.I.; Ussery, D.W. Defining the Pseudomonas genus: Where do we draw the line with Azotobacter? Microb. Ecol. 2012, 63, 239-248.
Pourahmad, M.; Javadi, A.; Kamran, A.; Ataei, B.; Yaran, M. and Jharomi, A.S., (2018). A clinical debate concerning aminoglycoside resistance genes among Pseudomonas aeruginosa strains. Mid. Eas. J. Fam.
Rajabi, H; Salimizand, H; Khodabandehloo, M; Fayyazi, A; Ramazanzadeh, R. (2022). Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains. Bio. Med. Res. Int. 1716087, 1-7.
Ranjbar, R., Owlia, P., Saderi, H., Mansouri, S., Jonaidi-Jafari, N., Izadi, M., Farshad, S., and Arjomandzadegan, M. (2011). Characterization of Pseudomonas aeruginosa strains isolated from burned patients hospitalized in a major burn center in Tehran, Iran. Acta medica Iranica. 49: 675-679.
Rodrigues, R, L, Lima, J, L, C, Sena, K, X, F, R, Maciel, M, A, V (2020). Phenotypic and genotypic analysis of biofilm production by Pseudomonas aeruginosa isolates from infection and colonization samples. Journal of the Brazilian Society of Tropical Medicine. Vol:53:(e20200399).
Shatti, H, H, Al- saeed, W, M, Nader, M, I. (2022). Effect Biofilm Formation in Pseudomonas aeruginosa Resistance to Antibiotic. Mustansiriya Medical Journal. vol: 21(2): 1-17.
Simanek, KA, Taylor IR, Richael EK, Lasek-Nesselquist E, Bassler BL, Paczkowski JE. (2022). The PqsE-RhlR interaction regulates RhlR DNA binding to control virulence factor production in Pseudomonas aeruginosa. Microbiology spectrum. 10(1): e02108-21.
Vaez, H.; Safei, H. G. and Faghri, J. (2017). The emergence of multidrug- resistant clone ST664 Pseudomonas aeruginosa in a referral burn hospital, Isfahan, Iran. Burns and Truma. 5(27):1-2.
Yang, F; Liu, C; Ji, J; Cao, W; Ding, B; Xu, X. (2021). Molecular Characteristics, Antimicrobial Resistance, and Biofilm Formation of Pseudomonas aeruginosa Isolated from Patients with Aural Infections in Shanghai, China. Inf. Dru. Res. 14:3637–3645.
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