Identification study of soil fungi in different areas of Diyala governorate

Authors

DOI:

https://doi.org/10.24237/04.01.612

Keywords:

soil fungi,, Morphologies, Aspergillus, Czapek Dox Medium (CDA).

Abstract

The present study dealt with the isolation and characterization of fungi from 33 different soil samples from Diyala governorate, including agricultural soil, residential area soil, sanitary landfill area soil and industrial area soil. The fungi have been isolated directly from the soil dilution method using Sabouraud’s dextrose agar (SDA). The grown fungi colonies have then been subcultured on Czapek Dox Medium (CDA) for identification; also, the fungi have been examined microscopically with lactophenol cotton blue stain. The results showed that 87 fungal isolates of 25 species have been identified in 33 locations, categorised over four types of soil samples, where most of the samples were from agricultural areas, followed by residential areas, then sanitary landfill areas and industrial areas, and the results have shown that the highest percentage of fungi isolates was Aspergillus flavus with 14 (16.09%) at agricultural and residential areas with 14.54% and 16.66 % respectively, followed by Aspergillus niger with 12 (13.79%) The third one in the isolation percentage was Trichoderma sp. with 8 (9.19%). Other species were in lower percentages, with the presence of pathogens that are pathogenic to humans and animals.

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Author Biographies

  • Dr. Anaam Fouad Hussein, University of Diyala

    Dr. Anaam Fouad Hussein is a Professor in the Department of Biotechnology, College of Science, University of Diyala, Iraq. She holds a PhD in Mycology from the University of Baghdad. Her academic interests focus on fungal biology and biotechnology, with experience in university teaching, scientific research, and supervision of undergraduate and postgraduate students. He can be contacted at email: anaamfuad@Uodiyala.edu.iq

  • Dr. Abbas Muhi Mazhar, University of Diyala

    Dr. Abbas Muhi Mazhar is an Assistant Professor specializing in Mycology (Fungal Biology) at the College of Science – University of Diyala, where he serves in the Department of Forensic Evidence. His academic interests focus on the theoretical and applied aspects of mycology, particularly in areas relevant to forensic and scientific investigations. He is actively involved in teaching and academic research, contributing to the advancement of scientific knowledge and higher education. He can be contacted at email: abbas@uodiyala.edu.iq

  • Mujtaba Ahmed A. Hussein

    Mujtaba Ahmed A. Hussein An academic researcher specialized in biotechnology and applied biological sciences, with research interests focused on microbiome studies, probiotics, and modern biomedical applications, and with peer-reviewed publications in international indexed journals. He can be contacted at email: mujtaba.ahmed@mm-edu.in

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Published

2026-01-30

How to Cite

anaam, Abbas, & Mujtaba. (2026). Identification study of soil fungi in different areas of Diyala governorate. ٍِASJ - Academic Science Journal, 4(1), 59-67. https://doi.org/10.24237/04.01.612

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